Circulating SARS-CoV-2 Vaccine Antigen Detected in the Plasma of mRNA-1273 Vaccine Recipients

Abstract

SARS-CoV-2 proteins were measured in longitudinal plasma samples collected from 13 participants who received two doses of mRNA-1273 vaccine. 11 of 13 participants showed detectable levels of SARS-CoV-2 protein as early as day one after first vaccine injection. Clearance of detectable SARS-CoV-2 protein correlated with production of IgG and IgA.


 https://academic.oup.com/cid/advance-article/doi/10.1093/cid/ciab465/6279075?fbclid=IwAR22xjnzsC0ayKSA5Z63CReAXzK2Od0M8P0Rx-Yq6n3Pwteopl0amqU1G-o


Q. Do Vaccines Disappear in Body after  2-3 Days? 

A. This submitted and accepted paper shows at least 30 days


Q. Is this a big study

A. No Only 13 people. 


Things to note: S1 antigen was detected as early as day one post vaccination and peak levels were detected on average five days after the first injection. 


For one individual (Participant #8), spike was detected at day 29, one day after the second injection and was undetectable two days later. 


Summary:

Here, we provide evidence that circulating SARS-CoV-2 proteins are present in the plasma of participants vaccinated with the mRNA-1273 vaccine. We report antigen and serological data of the mRNA-1273 vaccine in 13 healthcare workers at the Brigham and Women’s Hospital. Ultrasensitive single-molecule array (Simoa) assays were used for the detection of SARS-CoV-2 antigens spike (S1-S2 unit), S1, and nucleocapsid and antibodies IgG, IgA, and IgM against SARS-CoV-2 spike, S1, receptor binding domain (RBD), and

6,7

nucleocapsid, as previously described
enable detection of antigen and antibody production in the early stages post vaccination and quantification of changes in levels over the course of both vaccine injections.


Comments

Post a Comment

Popular posts from this blog

Transmission in the Vaccinated: Vietnam Hospital Worker Study PREPRINT

Image
 https://papers.ssrn.com/sol3/papers.cfm?abstract_id=3897733 Abstract Background: Data on breakthrough SARS-CoV-2 Delta variant infections are limited. Methods: We studied breakthrough infections among healthcare workers of a major infectious diseases hospital in Vietnam. We collected demographics, vaccination history and results of PCR diagnosis alongside clinical data. We measured SARS-CoV-2 (neutralizing) antibodies at diagnosis, and at week 1, 2 and 3 after diagnosis. We sequenced the viruses using ARTIC protocol. Findings: Between 11th–25th June 2021 (week 7–8 after dose 2), 69 healthcare workers were tested positive for SARS-CoV-2. 62 participated in the clinical study. 49 were (pre)symptomatic with one requiring oxygen supplementation. All recovered uneventfully. 23 complete-genome sequences were obtained. They all belonged to the Delta variant, and were phylogenetically distinct from the contemporary Delta variant sequences obtained from community transmission cases, sugges...
Read more

Leaky Vaccines Create Variants-The Vaccinated Infect the Unvaccinated

 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4516275/ RESEARCH ARTICLE Imperfect Vaccination Can Enhance the Transmission of Highly Virulent Pathogens Andrew F. Read1,2*, Susan J. Baigent3 , Claire Powers3 , Lydia B. Kgosana3 , Luke Blackwell3 , Lorraine P. Smith3 , David A. Kennedy1,2, Stephen W. Walkden-Brown4 , Venugopal K. Nair3 1 Center for Infectious Disease Dynamics, Departments of Biology and Entomology, The Pennsylvania State University, University Park, Pennsylvania, United States of America, 2 Fogarty International Center, National Institutes of Health, Bethesda, Maryland, United States of America, 3 Avian Oncogenic Virus Group, The Pirbright Institute, Compton, Newbury, Berkshire, United Kingdom, 4 School of Environmental and Rural Science, The University of New England, Armidale, Australia * a.read@psu.edu Abstract Could some vaccines drive the evolution of more virulent pathogens? Conventional wisdom is that natural selection will remove highly lethal pathogens if ho...
Read more

Vaccines Spike Protein: Risk of rapid evolutionary escape from biomedical interventions targeting SARS-CoV-2 spike protein

Image
 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0250780 The spike protein receptor-binding domain (RBD) of SARS-CoV-2 is the molecular target for many vaccines and antibody-based prophylactics aimed at bringing COVID-19 under control. Such a narrow molecular focus raises the specter of viral immune evasion as a potential failure mode for these biomedical interventions. With the emergence of new strains of SARS-CoV-2 with altered transmissibility and immune evasion potential, a critical question is this: how easily can the virus escape neutralizing antibodies (nAbs) targeting the spike RBD? To answer this question, we combined an analysis of the RBD structure-function with an evolutionary modeling framework. Our structure-function analysis revealed that epitopes for RBD-targeting nAbs overlap one another substantially and can be evaded by escape mutants with ACE2 affinities comparable to the wild type, that are observed in sequence surveillance data and infect cel...
Read more